The Brazilian ineins used in this research will be prepared according to the procedure in the previously reported research (Rondão et al., 2013). Start by soaking rough lygwood (about a toothpick size) 500 g. In the methanol, the volume of 1,800 ml for 3 days, then filter the fang spat out of the solution through thin whites and filter using filter paper, and then evaporate the dissolved body using a distillation machine, rotating under the vacuum until the water is reduced to approximately 150 ml. Wash the sediment with cold pure methanol, and dry the sediment will be stored in the next experiment.
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